micro-spotting solution plus 2 Search Results


transmission electron microscope  (JEOL)
99
JEOL transmission electron microscope
Figure 5. Supravital cell staining with acridine orange using a fluorescence <t>microscope</t> revealed that the induction of acidic vesicular organelles (AVOs) and autophagy are associated with changes in the microtubule-associated pro- tein 1 light chain 3 (LC3). (A) Microphotograph of AVOs in U118-MG cells. Detection of green and red fluorescence in acridine orange-stained cells was performed using a fluorescence microscope. The white arrows point to AVOs. (B) EM microphotographs of U118-MG cells treated with IR and ATO alone or in combination. Cells were pretreated with IR (4 Gy) and cultured for 18 hours, then treated with ATO (2 μM) for 12 hours. The times of individual treatment with IR or ATO were 30 and 12 hours, respectively. The white arrows point to autophagic vacuoles and autolyso- somes. Bars: 2 μm (Control); 1 μm (IR, ATO, IR + ATO); 500 nm (ATO/2); 200 nm (IR + ATO/2). (C) Time-course of LC3-I and LC3-II protein expression in U118-MG cells treated with IR and ATO alone or in combination. In the combined treatment, cells were pretreated with IR, and cultured for 18 hours and then treated with ATO for 6, 12 and 18 hours.
Transmission Electron Microscope, supplied by JEOL, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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antigen unmasking solution  (Vector Laboratories)
96
Vector Laboratories antigen unmasking solution
Figure 5. Supravital cell staining with acridine orange using a fluorescence <t>microscope</t> revealed that the induction of acidic vesicular organelles (AVOs) and autophagy are associated with changes in the microtubule-associated pro- tein 1 light chain 3 (LC3). (A) Microphotograph of AVOs in U118-MG cells. Detection of green and red fluorescence in acridine orange-stained cells was performed using a fluorescence microscope. The white arrows point to AVOs. (B) EM microphotographs of U118-MG cells treated with IR and ATO alone or in combination. Cells were pretreated with IR (4 Gy) and cultured for 18 hours, then treated with ATO (2 μM) for 12 hours. The times of individual treatment with IR or ATO were 30 and 12 hours, respectively. The white arrows point to autophagic vacuoles and autolyso- somes. Bars: 2 μm (Control); 1 μm (IR, ATO, IR + ATO); 500 nm (ATO/2); 200 nm (IR + ATO/2). (C) Time-course of LC3-I and LC3-II protein expression in U118-MG cells treated with IR and ATO alone or in combination. In the combined treatment, cells were pretreated with IR, and cultured for 18 hours and then treated with ATO for 6, 12 and 18 hours.
Antigen Unmasking Solution, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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micro-spotting solution plus 2  (Arrayit Corporation)
90
Arrayit Corporation micro-spotting solution plus 2
Figure 5. Supravital cell staining with acridine orange using a fluorescence <t>microscope</t> revealed that the induction of acidic vesicular organelles (AVOs) and autophagy are associated with changes in the microtubule-associated pro- tein 1 light chain 3 (LC3). (A) Microphotograph of AVOs in U118-MG cells. Detection of green and red fluorescence in acridine orange-stained cells was performed using a fluorescence microscope. The white arrows point to AVOs. (B) EM microphotographs of U118-MG cells treated with IR and ATO alone or in combination. Cells were pretreated with IR (4 Gy) and cultured for 18 hours, then treated with ATO (2 μM) for 12 hours. The times of individual treatment with IR or ATO were 30 and 12 hours, respectively. The white arrows point to autophagic vacuoles and autolyso- somes. Bars: 2 μm (Control); 1 μm (IR, ATO, IR + ATO); 500 nm (ATO/2); 200 nm (IR + ATO/2). (C) Time-course of LC3-I and LC3-II protein expression in U118-MG cells treated with IR and ATO alone or in combination. In the combined treatment, cells were pretreated with IR, and cultured for 18 hours and then treated with ATO for 6, 12 and 18 hours.
Micro Spotting Solution Plus 2, supplied by Arrayit Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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superfrost plus micro slides  (Avantor)
90
Avantor superfrost plus micro slides
Figure 5. Supravital cell staining with acridine orange using a fluorescence <t>microscope</t> revealed that the induction of acidic vesicular organelles (AVOs) and autophagy are associated with changes in the microtubule-associated pro- tein 1 light chain 3 (LC3). (A) Microphotograph of AVOs in U118-MG cells. Detection of green and red fluorescence in acridine orange-stained cells was performed using a fluorescence microscope. The white arrows point to AVOs. (B) EM microphotographs of U118-MG cells treated with IR and ATO alone or in combination. Cells were pretreated with IR (4 Gy) and cultured for 18 hours, then treated with ATO (2 μM) for 12 hours. The times of individual treatment with IR or ATO were 30 and 12 hours, respectively. The white arrows point to autophagic vacuoles and autolyso- somes. Bars: 2 μm (Control); 1 μm (IR, ATO, IR + ATO); 500 nm (ATO/2); 200 nm (IR + ATO/2). (C) Time-course of LC3-I and LC3-II protein expression in U118-MG cells treated with IR and ATO alone or in combination. In the combined treatment, cells were pretreated with IR, and cultured for 18 hours and then treated with ATO for 6, 12 and 18 hours.
Superfrost Plus Micro Slides, supplied by Avantor, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tissue-tek slide staining set  (Avantor)
90
Avantor tissue-tek slide staining set
Figure 5. Supravital cell staining with acridine orange using a fluorescence <t>microscope</t> revealed that the induction of acidic vesicular organelles (AVOs) and autophagy are associated with changes in the microtubule-associated pro- tein 1 light chain 3 (LC3). (A) Microphotograph of AVOs in U118-MG cells. Detection of green and red fluorescence in acridine orange-stained cells was performed using a fluorescence microscope. The white arrows point to AVOs. (B) EM microphotographs of U118-MG cells treated with IR and ATO alone or in combination. Cells were pretreated with IR (4 Gy) and cultured for 18 hours, then treated with ATO (2 μM) for 12 hours. The times of individual treatment with IR or ATO were 30 and 12 hours, respectively. The white arrows point to autophagic vacuoles and autolyso- somes. Bars: 2 μm (Control); 1 μm (IR, ATO, IR + ATO); 500 nm (ATO/2); 200 nm (IR + ATO/2). (C) Time-course of LC3-I and LC3-II protein expression in U118-MG cells treated with IR and ATO alone or in combination. In the combined treatment, cells were pretreated with IR, and cultured for 18 hours and then treated with ATO for 6, 12 and 18 hours.
Tissue Tek Slide Staining Set, supplied by Avantor, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Figure 5. Supravital cell staining with acridine orange using a fluorescence microscope revealed that the induction of acidic vesicular organelles (AVOs) and autophagy are associated with changes in the microtubule-associated pro- tein 1 light chain 3 (LC3). (A) Microphotograph of AVOs in U118-MG cells. Detection of green and red fluorescence in acridine orange-stained cells was performed using a fluorescence microscope. The white arrows point to AVOs. (B) EM microphotographs of U118-MG cells treated with IR and ATO alone or in combination. Cells were pretreated with IR (4 Gy) and cultured for 18 hours, then treated with ATO (2 μM) for 12 hours. The times of individual treatment with IR or ATO were 30 and 12 hours, respectively. The white arrows point to autophagic vacuoles and autolyso- somes. Bars: 2 μm (Control); 1 μm (IR, ATO, IR + ATO); 500 nm (ATO/2); 200 nm (IR + ATO/2). (C) Time-course of LC3-I and LC3-II protein expression in U118-MG cells treated with IR and ATO alone or in combination. In the combined treatment, cells were pretreated with IR, and cultured for 18 hours and then treated with ATO for 6, 12 and 18 hours.

Journal: Autophagy

Article Title: Combination treatment with arsenic trioxide and irradiation enhances autophagic effects in U118-MG cells through increased mitotic arrest and regulation of PI3K/Akt and ERK1/2 signaling pathways.

doi: 10.4161/auto.5.4.7759

Figure Lengend Snippet: Figure 5. Supravital cell staining with acridine orange using a fluorescence microscope revealed that the induction of acidic vesicular organelles (AVOs) and autophagy are associated with changes in the microtubule-associated pro- tein 1 light chain 3 (LC3). (A) Microphotograph of AVOs in U118-MG cells. Detection of green and red fluorescence in acridine orange-stained cells was performed using a fluorescence microscope. The white arrows point to AVOs. (B) EM microphotographs of U118-MG cells treated with IR and ATO alone or in combination. Cells were pretreated with IR (4 Gy) and cultured for 18 hours, then treated with ATO (2 μM) for 12 hours. The times of individual treatment with IR or ATO were 30 and 12 hours, respectively. The white arrows point to autophagic vacuoles and autolyso- somes. Bars: 2 μm (Control); 1 μm (IR, ATO, IR + ATO); 500 nm (ATO/2); 200 nm (IR + ATO/2). (C) Time-course of LC3-I and LC3-II protein expression in U118-MG cells treated with IR and ATO alone or in combination. In the combined treatment, cells were pretreated with IR, and cultured for 18 hours and then treated with ATO for 6, 12 and 18 hours.

Article Snippet: The cells were fixed with a solution containing 2.5% glutaraldehyde plus 2% paraformaldehyde in 0.1 M cacodylate buffer, pH 7.3, for 1 h. After fixation, the samples were postfixed in 1% OsO4 in the same buffer for 30 min. Ultra-thin sections were then observed under a transmission electron microscope (JEOL JEM-1200EX, Japan) at 100 kV.

Techniques: Staining, Fluorescence, Microscopy, Cell Culture, Control, Expressing